Click Chemistry Reagents

Click chemistry continues to gain popularity and is used in a variety of research fields with significant contributions to the fields of chemical biology, polymer chemistry, bioconjugation, and drug discovery. Our extensive portfolio of click chemistry reagents offers a variety of azides, alkynes, cyclic alkynes (DBCO and BCN), Tetrazine/TCO, and ligands to help accelerate your research in the exciting arena of “click” chemistry. We can customize different linkers (PEG linker, peptide linker etc) to find the best click reagents that work for your application.

Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC): CuAAC is a cornerstone of "click" chemistry, involving the reaction of azides with terminal alkynes in the presence of a copper(I) catalyst to form 1,2,3-triazoles. Renowned for its efficiency, selectivity, and versatility, CuAAC has broad applications across chemistry and biology. However, it is not without limitations; challenges include potential copper toxicity in biological systems and the difficulty of removing residual copper from final products. These issues are particularly significant in pharmaceutical and sensitive biomolecular applications, necessitating strategies to mitigate copper-related concerns.

Strain-promoted alkyne-azide cycloaddition (SPAAC): SPAAC has become a prominent bioorthogonal reaction, widely utilized in fields such as chemical biology, drug discovery, and materials science. This reaction stands out for its exceptional efficiency, speed, selectivity, and compatibility with living systems. Unlike other reactions, SPAAC does not require external stimuli such as light, heat, or catalysts. Its mechanism leverages the inherent strain energy of cyclic alkynes, which react readily with azides. Over time, diverse cyclooctyne derivatives have been engineered and optimized to enhance SPAAC reactivity and broaden its applications.

Inverse Electron Demand Diels–Alder (IEDDA) reaction: IEDDA is recognized for its unmatched kinetics, superior orthogonality, and biocompatibility, making it a standout among bioorthogonal reactions. An exemplary IEDDA reaction, known as TCO ligation and pioneered by Fox et al., entails the reaction of an electron-deficient tetrazine (Tz) with a strained trans-cyclooctene (TCO) derivative, making it an ideal tool for in vivo applications. Through collaborative licensing with Professor Fox's group, we are proud to offer a specialized selection of unique TCO and tetrazine compounds tailored to support your research needs.