DBCO PEG

The strain-promoted alkyne-azide cycloaddition (SPAAC) has gained widespread adoption as a bioorthogonal reaction, finding extensive applications across diverse fields due to its distinguished attributes of high efficiency, rapidity, selectivity, and bioorthogonality. Notably, SPAAC reactions do not require additional stimuli such as light, heat, ultrasound, or catalysts. The impetus for this reaction originates from the intrinsic strain present in the active cyclic alkynes. Over time, various cyclooctyne derivatives have been developed and meticulously examined for their reactivity in SPAAC reactions with azides.

DBCO PEG represents a specific category of PEG linker featuring a highly reactive DBCO (Dibenzocyclooctyne) group. DBCO exhibits the capability to react with azide groups without a catalyst through a strain-promoted click reaction. This reaction can be conducted either in an organic solvent or under mild buffer conditions and holds the advantage of not affecting other functional groups present in biological samples. DBCO is instrumental for bioconjugation in live cells, non-living samples, and whole organisms.

Exemplary DBCO PEG products include:

1. DBCO-PEG4-DBCO: A PEG linker characterized by two DBCO groups and a hydrophilic PEG spacer arm.

2. DBCO-PEG4-amido Mal: A bioorthogonal linker featuring azide and sulfhydryl reactivity, with distinct functional groups at each end.

DBCO-NHCO-PEG-Amido MaI

DBCO-NHCO-PEG-Amine

DBCO-NHCO-PEG-Methyl Ester

DBCO-NHCO-PEG-NHS Ester

DBCO-NHCO-PEG-t-Boc Amine